Subtract

Author

Cox Lab

Published

March 18, 2024

1 General

  • Type: - Matrix Processing
  • Heading: - Normalization
  • Source code: Subtract.cs

2 Brief description

The specified quantity calculated on each row/column is subtracted from each value.

Output: Normalized expression matrix.

3 Parameters

3.1 Matrix access

Specifies whether the subtraction is performed on rows or columns (default: Rows). If the subtraction is performed on rows, the “Grouping” can be specified as well (default: ).

3.1.1 Grouping

This parameter is just relevant, if the “Matrix access” is set to “Rows”. It specifies, whether the normalization should be applied separately on groups (default: ).

3.2 Subtract what

Defines what value should be subtracted from all entries in expression columns (default: Median). The subtrahend can be selected from a predefined list:

  • Mean
  • Median
  • Most frequent value
  • Tukey’s biweight

4 Parameter window